human breast cancer cells mda-m231 Search Results


human breast cancer cells mda-m231  (Korean Cell Line Bank)
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Korean Cell Line Bank human breast cancer cells mda-m231
( a–f ) Intracellular fluorescence intensity recorded <t>for</t> <t>A549</t> cells 1 h after incubation with of PT-1 in the absence ( a–c ) of and ( d–f ) after irradiation with 405 nm light for 1 h over the area delineated by the yellow dashed line. ( g ) Cytotoxicity of PT-1 observed in two cancer cell lines (A549 and <t>HeLa)</t> and two human normal fibroblast cell lines (WI-38 and BJ) after photo-activation. ( h ) Cell viability (relative percentage) observed for the A549, HeLa, WI-38, and BJ cell lines after treatment with PT-1 (incubation for 1 h) followed by irradiation at 405 nm for 1 h. Viability was determined via a 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay performed 24 h after photo-irradiation. *P < 0.05. ( i–l ) Phase contrast images of A549 cancer cells undergoing apoptosis. In these studies, the cells were incubated with 10 nM PT-1 for 30 min followed by irradiation with 405 nm laser light for 1 h over the area indicated by the yellow dashed lines ( j , l ).
Human Breast Cancer Cells Mda M231, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( a–f ) Intracellular fluorescence intensity recorded for A549 cells 1 h after incubation with of PT-1 in the absence ( a–c ) of and ( d–f ) after irradiation with 405 nm light for 1 h over the area delineated by the yellow dashed line. ( g ) Cytotoxicity of PT-1 observed in two cancer cell lines (A549 and HeLa) and two human normal fibroblast cell lines (WI-38 and BJ) after photo-activation. ( h ) Cell viability (relative percentage) observed for the A549, HeLa, WI-38, and BJ cell lines after treatment with PT-1 (incubation for 1 h) followed by irradiation at 405 nm for 1 h. Viability was determined via a 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay performed 24 h after photo-irradiation. *P < 0.05. ( i–l ) Phase contrast images of A549 cancer cells undergoing apoptosis. In these studies, the cells were incubated with 10 nM PT-1 for 30 min followed by irradiation with 405 nm laser light for 1 h over the area indicated by the yellow dashed lines ( j , l ).

Journal: Scientific Reports

Article Title: Programmed activation of cancer cell apoptosis: A tumor-targeted phototherapeutic topoisomerase I inhibitor

doi: 10.1038/srep29018

Figure Lengend Snippet: ( a–f ) Intracellular fluorescence intensity recorded for A549 cells 1 h after incubation with of PT-1 in the absence ( a–c ) of and ( d–f ) after irradiation with 405 nm light for 1 h over the area delineated by the yellow dashed line. ( g ) Cytotoxicity of PT-1 observed in two cancer cell lines (A549 and HeLa) and two human normal fibroblast cell lines (WI-38 and BJ) after photo-activation. ( h ) Cell viability (relative percentage) observed for the A549, HeLa, WI-38, and BJ cell lines after treatment with PT-1 (incubation for 1 h) followed by irradiation at 405 nm for 1 h. Viability was determined via a 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay performed 24 h after photo-irradiation. *P < 0.05. ( i–l ) Phase contrast images of A549 cancer cells undergoing apoptosis. In these studies, the cells were incubated with 10 nM PT-1 for 30 min followed by irradiation with 405 nm laser light for 1 h over the area indicated by the yellow dashed lines ( j , l ).

Article Snippet: Twelve biotin receptor-positive cell lines; human lung carcinoma cells (A549), human cervical cancer cells (HeLa), human breast cancer cells (MCF7, MDA-M231), human liver cancer cells (HepG2, Huh7, Hep3B), human prostate cancer cells (Du145, PC3), human gastric cancer cells (NCI-N87, AGS), human pancreatic cancer cell (Panc-1), and a biotin receptor-negative cell: human normal embryonal kidney epithelial cell (293T) were purchased from the Korean Cell Line Bank (Seoul, Republic of Korea).

Techniques: Fluorescence, Incubation, Irradiation, Activation Assay, MTT Assay